• Skip to primary navigation
  • Skip to main content
  • Skip to primary sidebar

Nouailles Lab - Immunobiology and Respiratory Infections

  • Home
  • News
  • Research
  • Funding
  • Publications
  • Team
    • Alumni
  • Career

Nouailles Lab

Nouailles Lab — Kopie

Nouailles Lab

Immunobiology and Respiratory Infections

Our research lab Immunobiology and Respiratory Infections is part of the Department of Infectious Diseases, Respiratory Medicine and Critical Care at the Charité – Universitätsmedizin Berlin.

The group is working on pulmonary and immunological defense mechanisms in response to bacterial and viral infections to understand capacities and limitations of the host defense. Learn more about our different approaches and research projects.

Alumni

Alumni

Sarah Berger

Wibke Grönewald

Alexander Taylor

Lisa Pfeiffer

Katharina Clausnitzer

Sophia Rotter

Career

Career

We are always looking for people joining our team. If you are interest, send Geraldine an email (geraldine.nouailles@charite.de).

Team

Our Team

Geraldine Nouailles

Group Leader, PD Dr.-Ing., she/her

Ulrike Behrendt

Lab technician, she/her

Romina Bischoff

DVM student, she/her

Cengiz Gökeri

Postdoc, Dr. rer. nat., he/him

Niall Hardiman

PhD student, he/him

Karen Hoffmann

Postdoc, Dr. rer. nat., she/her

Kerstin Linke

DVM student, she/her

Alina Nettesheim

MD student, she/her

Peter Pennitz

Postdoc, Dr. med., he/him

Sebastian Schickinger

MD student, he/him

Chantal Weißfuß

PhD student, she/her

Anne Angela Poletti

Master student, she/her

Our Group pictures

2024

2022

Publications

The complete bibliography of Geraldine Nouailles is available at PubMed.

You will find selected publications in the distinct research project descriptions


Research

Research

Find more about our research at the Nouailles Lab!

COVID-19 and Single-Cells

Single-cell sequencing and immunology reveal host responses to SARS-CoV-2 and other respiratory pathogens.

Lung Innate Immunity

Orchestration of chemokine expression and neutrophil responses in acute and chronic lung infections.

Lung Epithelial Glycocalyx

Respiratory epithelial glycocalyx and glycoproteins – host defense or entry point for pathogens.

Lung Models for Pneumonia Research

Developing 2D and 3D human lung models, xeno-free culturing and implementation of 3R principles.

Phages – Bacteria-Killing Viruses

Host immunity – innate pulmonary immunity booster or dampener of phage therapy efficacy.

Therapy and Mode of Action

Protective mode of action of immunoglobulin (Trimodulin) therapy in pneumonia. 

Systems Medicine

In vitro, in vivo and real-world data turned into mathematical models of pneumonia and cardiovascular complications thereof.

Single-cell sequencing and immunology reveal host responses to SARS-CoV-2 and other respiratory pathogens.

Project members

Geraldine Nouailles

Peter Pennitz

Ulrike Behrendt

Cengiz Gökeri

Main cooperation partners

Jakob Trimpert and team

Emanuel Wyler/Markus Landthaler and team

Holger Kirsten/Markus Scholz and team

Samantha Praktiknjo and team

Michael Mülleder and team

Achim Gruber and team

Christine Goffinet and team

…many more

Publications

Friedrich VD*, Pennitz P*, Wyler E, Adler JM, Postmus D, Müller K, Teixeira Alves LG, Prigann J, Pott F, Vladimirova D, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Saliba AE, Scholz M, Witzenrath M, Trimpert J, Kirsten H#, Nouailles G#. Neural network-assisted humanisation of COVID-19 hamster transcriptomic data reveals matching severity states in human disease. EBioMedicine. 2024;108:105312. *,#: equal contribution.

Peidli S*, Nouailles G*, Wyler E, Adler JM, Kunder S, Voß A, Kazmierski J, Pott F, Pennitz P, Postmus D, Teixeira Alves LG, Goffinet C, Gruber AD, Blüthgen N, Witzenrath M, Trimpert J#, Landthaler M#, Praktiknjo SD#. Single-cell-resolved interspecies comparison shows a shared inflammatory axis and a dominant neutrophil-endothelial program in severe COVID-19. Cell Rep. 2024;43(6):114328. *,#: equal contribution.

Adler JM, Martin Vidal R, Langner C, Vladimirova D, Abdelgawad A, Kunecova D, Lin X, Nouailles G, Voss A, Kunder S, Gruber AD, Wu H, Osterrieder N, Kunec D, Trimpert J. An intranasal live-attenuated SARS-CoV-2 vaccine limits virus transmission. Nat Commun. 2024;15(1):995.

Friedrich VD#, Pennitz P#, Wyler E, Adler JM, Postmus D, Teixeira Alves LG, Prigann J, Pott F, Vladimirova D, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Saliba AE, Scholz M, Witzenrath M, Trimpert J, Kirsten H*, Nouailles G*. Neural Network-Assisted Humanization of COVID-19 Hamster scRNAseq Data Reveals Matching Severity States in Human Disease. bioRxiv. 2024.01.11.574849. #shared first, *shared last

Peidli S#, Nouailles G#, Wyler E, Adler JM, Kinder S, Voß A, Kazmierski J, Pott F, Pennitz P, Postmus D, Teixeira Alves LG, Goffinet C, Gruber AD, Blüthgen N, Witzenrath M, Trimpert J*, Landthaler M*, Praktiknjo SD*. Single-cell-resolved interspecies comparison identifies a shared inflammatory axis and a dominant neutrophil-endothelial program in severe COVID-19. bioRxiv. 2023.08.25.551434. #shared first, *shared last

Nouailles G#, Adler JM#, Pennitz P, Peidli S, Alves LGT, Baumgardt M, Bushe J, Voss A, Langenhagen A, Langner C, Vidal RM, Pott F, Kazmierski J, Ebenig A, Lange MV, Mühlebach MD, Goekeri C, Simmons S, Xing N, Abdelgawad A, Herwig S, Cichon G, Niemeyer D, Drosten C, Goffinet C, Landthaler M, Blüthgen N, Wu H, Witzenrath M, Gruber AD, Praktiknjo SD, Osterrieder N, Wyler E*, Kunec D*, Trimpert J*. Live-attenuated vaccine sCPD9 elicits superior mucosal and systemic immunity to SARS-CoV-2 variants in hamsters. Nat Microbiol. 2023;8(5):860-874. #shared first, *shared last

Pennitz P, Goekeri C, Trimpert J, Wyler E, Ebenig A, Weissfuss C, Mühlebach MD, Witzenrath M, Nouailles G. Protocol to dissociate healthy and infected murine- and hamster-derived lung tissue for single-cell transcriptome analysis. STAR Protoc. 2022;4(1):101957

Pennitz P, Kirsten H, Friedrich VD, Wyler E, Goekeri C, Obermayer B, Heinz GA, Mashreghi MF, Büttner M, Trimpert J, Landthaler M, Suttorp N, Hocke AC, Hippenstiel S, Tönnies M, Scholz M, Kuebler WM, Witzenrath M, Hoenzke K, Nouailles G. A pulmonologist’s guide to perform and analyse cross-species single lung cell transcriptomics. Eur Respir Rev. 2022;31(165):220056.

Wyler E, Adler JM, Eschke K, Teixeira Alves G, Peidli S, Pott F, Kazmierski J, Michalick L, Kershaw O, Bushe J, Andreotti S, Pennitz P, Abdelgawad A, Postmus D, Goffinet C, Kreye J, Reincke SM, Prüss H, Blüthgen N, Gruber AD, Kuebler WM, Witzenrath M, Landthaler M, Nouailles G, Trimpert J. Key benefits of dexamethasone and antibody treatment in COVID-19 hamster models revealed by single-cell transcriptomics. Mol Ther. 2022;30(5):1952-1965.

Nouailles G, Wyler E, Pennitz P, Postmus D, Vladimirova D, Kazmierski J, Pott F, Dietert K, Muelleder M, Farztdinov V, Obermayer B, Wienhold SM, Andreotti S, Hoefler T, Sawitzki B, Drosten C, Sander LE, Suttorp N, Ralser M, Beule D, Gruber AD, Goffinet C, Landthaler M, Trimpert J, Witzenrath M. Temporal omics analysis in Syrian hamsters unravel cellular effector responses to moderate COVID-19. Nat Commun. 2021;12(1):4869.

Ebenig A, Muraleedharan S, Kazmierski J, Todt D, Auste A, Anzaghe M, Gömer A, Postmus D, Gogesch P, Niles M, Plesker R, Miskey C, Gellhorn Serra M, Breithaupt A, Hörner C, Kruip C, Ehmann R, Ivics Z, Waibler Z, Pfaender S, Wyler E, Landthaler M, Kupke A, Nouailles G, Goffinet C, Brown RJP, Mühlebach MD. Vaccine-associated enhanced respiratory pathology in COVID-19 hamsters after TH2-biased immunization. Cell Rep. 2022;40(7):111214.

Andreotti S, Altmüller J, Quedenau C, Borodina T, Nouailles G, Teixeira Alves LG, Landthaler M, Bieniara M, Trimpert J, Wyler E. De Novo-Whole Genome Assembly of the Roborovski Dwarf Hamster (Phodopus roborovskii) Genome: An Animal Model for Severe/Critical COVID-19. Genome Biol Evol. 2022;14(7):evac100.

Conference Papers

ERS Congress 2024, 07. – 11.09.2024, Vienna, Austria

Pennitz P*, Friedrich VD*, Wyler E, Adler JM, Postmus D, Teixeira Alves LG, Prigann J, Pott F, Vladimirova D, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Saliba AE, Scholz M, Witzenrath M, Trimpert J, Kirsten H#, Nouailles G#. Neural Network-Assisted Humanization of COVID-19 Hamster Transcriptomic Data Reveals Matching Interspecies Severity States. European Respiratory Journal 2024 64(suppl 68): PA5318; DOI: https://doi.org/10.1183/13993003.congress-2024.PA5318.

64. Kongress der Deutschen Gesellschaft für Pneumologie und Beatmungsmedizin e. V. 20. – 23.04.2024, Mannheim

Pennitz P*, Friedrich V*, Wyler E, Adler J, Postmus D, Andreotti S, Teixeira Alves L, Kazmierski J, Pott F, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Trimpert J, Scholz M, Witzenrath M, Kirsten H#, Nouailles G#. Interspecies analysis to dissect transcriptomic signatures of humans and hamsters in CVID19. Pneumologie 2024; 78(S 01): S115-S116. DOI: 10.1055/s-0044-1778987

ERS Congress 2023, 09. – 13.09.2023, Milan, Italy

Pennitz P*, Friedrich VD*, Wyler E, Adler JM, Postmus D, Andreotti S, Teixeira Alves LG, Kazmierski J, Pott F, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Trimpert J, Scholz M, Witzenrath M, Kirsten H#, Nouailles G#. Interspecies analysis to dissect transcriptomic signatures of humans and hamsters in COVID-19. European Respiratory Journal 2023 62 (suppl 67): PA2334; DOI: https://doi.org/10.1183/13993003.congress-2023.PA2334

63. Kongress der Deutschen Gesellschaft für Pneumologie und Beatmungsmedizin e. V. 29.03. – 01.04.2023, Düsseldorf.

Pennitz P*, Friedrich V*, Wyler E, Adler J, Postmus D, Andreotti S, Teixeira-Alves L, Kazmierski J, Pott F, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Trimpert J, Scholz M, Witzenrath M, Kirsten H#, Nouailles G#. Interspecies analysis to dissect cellular transcriptomic signatures of humans and hamsters in COVID-19. Pneumologie 2023; 77(S 01): S41-S42, DOI: 10.1055/s-0043-1760966.

SCOG Conference “Single Cell Omics in Clinical Applications”, 07 – 09.11.2022, Bonn.

Pennitz P *, Friedrich VD *, Wyler E, Adler JM, Postmus D, Andreotti S, Teixeira-Alves LG, Kazmierski J, Pott F, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Trimpert J, Scholz M, Witzenrath M, Kirsten H#, Nouailles G#. Interspecies analysis to dissect cellular transcriptomic signatures of humans and hamsters in COVID-19.

21st International Conference on Systems Biology, 08.-12.10.2022, Berlin.

Pennitz P*, Kirsten H*, Friedrich VD, Wyler E, Goekeri C, Obermayer B, Heinz GA, Mashreghi MF, Büttner M, Trimpert J, Landthaler M, Suttorp N, Hocke AC, Hippenstiel S, Tönnies M, Scholz M, Kuebler WM, Witzenrath M, Hoenzke K, Nouailles G. Integrational approaches for cross-species analysis of lung pathologies at single-cell resolution.

e:Med Meeting 2021, 20 – 22.09.2021, online.

Pennitz P, Kirsten H, Goekeri C, Behrendt U, Gutbier B, Heinz G, Mashreghi MF, Wienhold SM, Witzenrath M, Scholz M, Nouailles G. Single-cell RNA-seq for a murine pneumonia lung atlas improving pathomechanistical understanding of community acquired pneumonia.

Project links

Wie ein altbekannter Wirkstoff zum Gamechanger werden kann (Gemeinsame Pressemitteilung von Charité, MDC und FU Berlin, 13.04.2022)

Modell als Grundlage neuer therapeutischer Ansätze (Gemeinsame Pressemitteilung der Charité, des MDC und der FU Berlin, 11.08.2021).

Transregio SFB-TR 84 “Innate Immunity of the Lung: Mechanisms of Pathogen Attack and Host Defence in Pneumonia“

Press Releases

How AI is helping to bridge the research gap between animals and humans (idw, 25.09.2024)

A new approach to a Covid-19 nasal vaccine shows early promise (CNN, 03.04.2023).

Live nasal COVID-19 vaccine candidate beats intramuscular mRNA shot in hamsters (The Science Advisory Board, 03.04.2023).

Nasal vaccine to prevent COVID-19 passes first tests (upi.com, 03.04.2023).

Nasenimpfstoff gegen Corona erfolgreich getestet (aerzteblatt.de, 04.04.2023).

Kommt die Nasenspray-Impfung gegen Corona? (zdfheute, 04.04.2023).

Angeblich besser als mRNA-Vakzin – Corona-Nasenimpfstoff erfolgreich an Hamstern getestet (Welt, 04.04.2023).

Impfung per Nasenspray könnte Pandemie beenden (Berliner Zeitung, 18.05.2022)

Impfung über Nasenspray: Können nasale Lebendimpfstoffe die Pandemie beenden, Herr Wyler? (Tagesspiegel, 23.05.2022)

Corona-Impfung per Nasenspray: Schnief und Schluss (Zeit-Online, 19.05.2022)

Lebendimpfstoff aus Berlin erzeugt Schleimhautimmunität (Spektrum.de, 18.05.2022)

Nasale Corona-Impfstoffe: Hoffnung auf sterile Immunität (Deutschlandfunk, 18.05.2022)

sCPD9: a SARS-CoV-2 nasal vaccine candidate that may have superior efficacy (News Medical, Life Science, 19.05.2022)

Neues Nasenspray aus Berlin soll besser als RNA-Impfung sein (mdr.de, 19.05.2022)

Spray könnte besser wirken als Spritze (blick.ch, 20.05.2022)

Corona-Impfung: Forscher sehen Erfolge im Tierversuch (sueddeutsche.de, 18.05.2022)

Orchestration of chemokine expression and neutrophil responses in acute and chronic lung infections.

Project members

Geraldine Nouailles

Cengiz Gökeri

Ulrike Behrendt

Sarah Berger (Alumni)

Main cooperation partners

Anca Dorhoi

Stefan Kaufmann

Sarah Hedtrich and team

Publications

Fiocca Vernengo F, Röwekamp I, Boillot L, Caesar S, Dörner PJ, Tarnowski B, Gutbier B, Nouailles G, Fatykhova D, Hellwig K, Witzenrath M, Hocke AC, Klatt AB, Opitz B. Diabetes impairs IFNγ-dependent antibacterial defense in the lungs. Mucosal Immunol. 2025;18(2):431-440.

Berger S*, Goekeri C*, Pennitz P*, Gutbier B, Michalick L, Hoffmann K, Lopez-Rodriguez E, Gluhovic V, Wienhold SM, Behrendt U, Taylor A, Dietert K, Kirsten H, Kunder S, Mueller K, Weigel M, Hain T, Volkers SM, Weis S, Gruber AD, Sander LE, Kuebler WM, CAPSyS study group, Witzenrath M, Nouailles G. Neutrophil-chemoattractant CXCL5 induces lung barrier permeability in acute lung injury. bioRxiv. 2024. *shared first

Goekeri C, Pennitz P, Groenewald W, Behrendt U, Kirsten H, Zobel CM, Berger S, Heinz GA, Mashreghi M-F, Wienhold S-M, Dietert K, Dorhoi A, Gruber AD, Scholz M, Rohde G, Suttorp N, CAPNETZ Study Group, Witzenrath M*, Nouailles G*. MicroRNA-223 Dampens Pulmonary Inflammation during Pneumococcal Pneumonia. Cells. 2023;12(6):959. *shared last

Nouailles G, Dorhoi A, Koch M, Zerrahn J, Weiner J 3rd, Faé KC, Arrey F, Kuhlmann S, Bandermann S, Loewe D, Mollenkopf HJ, Vogelzang A, Meyer-Schwesinger C, Mittrücker HW, McEwen G, Kaufmann SH. CXCL5-secreting pulmonary epithelial cells drive destructive neutrophilic inflammation in tuberculosis. J Clin Invest. 2014;124(3):1268-82.

Dorhoi A, Iannaccone M, Maertzdorf J, Nouailles G, Weiner J 3rd, Kaufmann SH. Reverse translation in tuberculosis: neutrophils provide clues for understanding development of active disease. Front Immunol. 2014;5:36.

Arrey F, Löwe D, Kuhlmann S, Kaiser P, Moura-Alves P, Krishnamoorthy G, Lozza L, Maertzdorf J, Skrahina T, Skrahina A, Gengenbacher M, Nouailles G*, Kaufmann SHE*. Humanized Mouse Model Mimicking Pathology of Human Tuberculosis for in vivo Evaluation of Drug Regimens. Front Immunol. 2019;10:89. *shared last

Dorhoi A, Iannaccone M, Farinacci M, Faé KC, Schreiber J, Moura-Alves P, Nouailles G, Mollenkopf HJ, Oberbeck-Müller D, Jörg S, Heinemann E, Hahnke K, Löwe D, Del Nonno F, Goletti D, Capparelli R, Kaufmann SH. MicroRNA-223 controls susceptibility to tuberculosis by regulating lung neutrophil recruitment. J Clin Invest. 2013;123(11):4836-48.

Links

CAPSyS: Systemmedizin der ambulant erworbenen Pneumonie (BMBF)

Projektbeschreibung CAPSys (Universität Leipzig)

The alveolar glycocalyx is a sugar-rich fluid layer that sits above the epithelium in our lungs. Consisting of membrane-anchored and freely floating glycoproteins, glycosaminoglycans and proteoglycans, it is located at a critical position, where oxygen exchange takes place and microbes exert their pathogenicity. As a group, we seek to demonstrate the precise involvement of the epithelial glycocalyx in maintaining normal physiology of the lungs including regulation of barrier permeability and immune cell transmigration. On the flip side, microbes have evolved to utilize sugar-harboring structures as attachment points to initiate their virulence programs. Whether the glycocalyx acts as our friend or foe during microbe-triggered pulmonary inflammation remains to be solved.

Project members

Geraldine Nouailles

Cengiz Gökeri

Karen Hoffmann

Niall Hardiman

Ulrike Behrendt

Alina Nettesheim

Kerstin Linke

Sebastian Schickinger

Main cooperation partners

Martin Witzenrath

Peter Seeberger and team

Wolfgang Kübler and team

Matthias Ochs and team

Kevin Pagel and team

Publications

Goekeri C#, Linke KAK#, Hoffmann K, Lopez-Rodriguez E, Gluhovic V, Voß A, Kunder S, Zappe A, Timm S, Nettesheim A, Schickinger SMK, Zobel CM, Pagel K, Gruber AD, Ochs M, Witzenrath M, Nouailles G. Enzymatic Modulation of the Pulmonary Glycocalyx Enhances Susceptibility to Streptococcus pneumoniae. Am J Respir Cell Mol Biol. 2024;71(6):646-658. #shared first.

Goekeri C#, Linke KAK#, Hoffmann K, Lopez-Rodriguez E, Gluhovic, Voß A, Kunder S, Zappe A, Timm S, Nettesheim A, Schickinger SMK, Zobel CM, Pagel K, Gruber AD, Ochs M, Witzenrath M, Nouailles G. Enzymatic modulation of the pulmonary glycocalyx alters susceptibility to Streptococcus pneumoniae. bioRxiv. 2024.01.03.573996. #shared first.

Herbst CJ, Lopez-Rodriguez E, Gluhovic V, Schulz S, Brandt R, Timm S, Abledu J, Falivene J, Pennitz P, Kirsten H, Nouailles G, Witzenrath M, Ochs M, Kuebler WM. Characterization of Commercially Available Human Primary Alveolar Epithelial Cells. Am J Respir Cell Mol Biol. 2024;70(5):339-350.

Timm S, Lettau M, Hegermann J, Rocha ML, Weidenfeld S, Fatykhova D, Gutbier B, Nouailles G, Lopez-Rodriguez E, Hocke A, Hippenstiel S, Witzenrath M, Kuebler WM, Ochs M. The unremarkable alveolar epithelial glycocalyx: a thorium dioxide-based electron microscopic comparison after heparinase or pneumolysin treatment. Histochem Cell Biol. 2023;160(2):83-96.

Ochs M, Hegermann J, Lopez-Rodriguez E, Timm S, Nouailles G, Matuszak J, Simmons S, Witzenrath M, Kuebler WM. On Top of the Alveolar Epithelium: Surfactant and the Glycocalyx. Int J Mol Sci. 2020;21(9):3075.

Conference Papers

Jenner Glycobiology and Medicine Symposium 2025, 10. – 13 June 2025, Maynooth, Ireland

Goekeri C, Schickinger S, Nettesheim A, Linke K, Bechtella L, Vos G, Lopez-Rodriguez E, Gluhovic V, Voß A, Kunder S, Bath J, Ribbeck K, Pagel K, Gruber A, Ochs M, Seeberger P, Witzenrath M, Nouailles G. Unraveling the Role of Terminal Fucosylation in Pneumococcal Pneumonia

Lung Science Conference (ERS), 20. – 23 March 2025, Estoril, Portugal

Goekeri C, Linke K, Hoffmann K, Lopez-Rodriguez E, Gluhovic V, Voß A, Kunder S, Zappe A, Timm S, Nettesheim A, Schickinger S, Zobel C, Bechtella L, Vos G, Pagel K, Gruber A, Ochs M, Witzenrath M, Nouailles G. Pulmonary glycosaminoglycans and fucosylation regulate lung barrier function and defense against S.pneumoniae

28th Joint Symposium: Infection and Immune Defense, 24. – 26 March 2025, Burg Rothenfels, Germany

Goekeri C, Schickinger S, Nettesheim A, Linke K, Bechtella L, Vos G, Lopez-Rodriguez E, Gluhovic V, Voß A, Kunder S, Bath J, Ribbeck K, Pagel K, Gruber A, Ochs M, Seeberger P, Witzenrath M, Nouailles G. Inhibition of pulmonary fucosylation alleviates pneumococcal pneumonia

28th Joint Symposium: Infection and Immune Defense, 24. – 26 March 2025, Burg Rothenfels, Germany

Hardiman N, Goekeri C, Linke K, Hoffmann K, Lopez-Rodriguez E, Gluhovic V, Voß A, Kunder S, Zappe A, Timm S, Nettesheim A, Schickinger S, Zobel C, Pagel K, Gruber A, Ochs M, Witzenrath M, Nouailles G. Enzymatic Modulation of the Pulmonary Glycocalyx Enhances Susceptibility to Streptococcus pneumoniae

ERS Congress 2024, 7. – 11 September 2024, Vienna, Austria

Goekeri C,  Schickinger SMK, Nettesheim A, Linke KAK, Bechtella L, Vos G, Lopez-Rodriguez E, Gluhovic V, Voß A, Kunder S, Bath J, Ribbeck K, Pagel K, Gruber AD, Ochs M,  Seeberger PH, Witzenrath M, Nouailles G. Unraveling the role of α-(1,2)-fucosylation in bacterial pneumonia. European Respiratory Journal. 2024 64(suppl 68): PA2395

ATS Conference 2024, 17. – 22 May 2024, San Diego, USA

Goekeri C,  Nettesheim A,  Schickinger SMK, Linke KAK, Behrendt U,  Barthel D, Lopez-Rodriguez E, Gluhovic V,  Schmidt K,  Bechtella L, Voß A, Kunder S, Pagel K, Gruber AD,  Hedtrich S,  Ochs M,  Seeberger PH, Witzenrath M, Nouailles G. Elucidating the Role of Epithelial α-(1,2)-fucosylation in Pneumococcal Virulence. American Journal of Respiratory and Critical Care Medicine 2024;209:A4360

ERS International Congress 2023, 09.-13.09.2023, Milan

Goekeri C,  Nettesheim A,  Linke KAK, Schickinger SMK,  Behrendt U,  Barthel D, Pennitz P, Lopez-Rodriguez E, Gluhovic V,  Schmidt K,  Bechtella L, Voß A, Kunder S, Pagel K, Gruber AD,  Hedtrich S,  Ochs M,  Witzenrath M, Nouailles G. Deciphering the role of pulmonary α-(1,2)-fucosylation in pneumococcal pneumonia. Eur Respir J. Sep 2023, 62 (suppl 67) PA1098.

1st International Symposium of the CRC 1449 on “Dynamic hydrogels at biointerfaces”, 06.-07.10.2022, Berlin

Linke K, Goekeri C, Nettesheim A, Behrendt U, Lopez-Rodriguez E, Ochs M, Witzenrath M, and Nouailles G. – Epithelial glycocalyx and bacterial pneumonia.

1st International Symposium of the CRC 1449 on “Dynamic hydrogels at biointerfaces”, 06.-07.10.2022, Berlin

Nettesheim A, Linke K, Behrendt U, Barthel D, Lopez-Rodriguez E, Ochs M, Gorenflos López J L, Hackenberger C P R, Seeberger P H, Witzenrath M, Goekeri C, and Nouailles G. – Characterizing the role of fucosylation in bacterial pneumonia

Project Links

Highlights/Papers by Junior Investigators in December 2024 issue of AJRCMB

Editorial comment for Goekeri et al. 2024

CRC 1449 Dynamic Hydrogels at Biointerfaces

Complete announcement from the Tagesspiegel supplement, issue of 3.10.2022

Developing 2D and 3D human lung models, xeno-free culturing and implementation of 3R principles.

Project members

Geraldine Nouailles

Karen Hoffmann

Cengiz Gökeri

Ulrike Behrendt

Lisa Pfeiffer

Peter Pennitz

Main cooperation partners

Christian Zobel, Werner Wenzel and team

Project-related publications

Goekeri C#, Linke KAK#, Hoffmann K, Lopez-Rodriguez E, Gluhovic V, Voß A, Kunder S, Zappe A, Timm S, Nettesheim A, Schickinger SMK, Zobel CM, Pagel K, Gruber AD, Ochs M, Witzenrath M, Nouailles G. Enzymatic Modulation of the Pulmonary Glycocalyx Enhances Susceptibility to Streptococcus pneumoniae. Am J Respir Cell Mol Biol. 2024;71(6):646-658. # shared first

Hoffmann K, Obermayer B, Hönzke K, Fatykhova D, Demir Z, Löwa A, Alves LGT, Wyler E, Lopez-Rodriguez E, Mieth M, Baumgardt M, Hoppe J, Firsching TC, Tönnies M, Bauer TT, Eggeling S, Tran HL, Schneider P, Neudecker J, Rückert JC, Gruber AD, Ochs M, Landthaler M, Beule D, Suttorp N, Hippenstiel S, Hocke AC, Kessler M. Human alveolar progenitors generate dual lineage bronchioalveolar organoids. Commun Biol. 2022;5(1):875.

Hoffmann K, Berger H, Kulbe H, Thillainadarasan S, Mollenkopf HJ, Zemojtel T, Taube E, Darb-Esfahani S, Mangler M, Sehouli J, Chekerov R, Braicu EI, Meyer TF, Kessler M. Stable expansion of high-grade serous ovarian cancer organoids requires a low-Wnt environment. EMBO J. 2020;39(6):e104013.

Conference Papers

EMBL – Infection: pathogens, hosts, and microbiomes, EMBO, 26. – 29 May 2025, Heidelberg, Germany.

Hoffmann K, Goekeri C, Behrendt U, Pohl J, Lopez-Rodriguez E, Weissfuss C, Kollmeier J, Tönnies M, Brill S, Steinestel K, Witzenrath M, Wenzel W, Zobel C, Nouailles G. Pneumonia-on-a-chip models highlight the crucial role of the glycocalyx and mechanical strain in regulating susceptibility to bacterial infections.

Human 3D Organ Models Networking Event of Charité 3R, 14 November 2024, Berlin, Germany

Hoffmann K, Weißfuß C, Goekeri C, Zobel C, Witzenrath M, Nouailles G.– In vitro models to study bacterial pneumonia.

DZL Annual Meeting, 05.06. – 07.06.2024, Bad Nauheim

Goekeri C, Linke KAK, Hoffmann K, Lopez-Rodriguez E, Gluhovic V, Voß A, Kunder S, Zappe A, Timm S, Nettesheim A, Schickinger SMK, Zobel CM, Pagel K, Gruber AD, Ochs M, Witzenrath M, Geraldine Nouailles. Enzymatic modulation of the pulmonary glycocalyx alters susceptibility to Streptococcus pneumoniae.

Si-Mposium, 12.09.2024, Berlin

Hoffmann K, Goekeri C, Zobel CM, Witzenrath M, Nouailles G. Alveolus-on-a-chip for pneumonia research.

ERS Congress, 07.09. – 11.09.2024, Vienna

Hoffmann K, Goekeri C, Obermayer-Wasserscheid B, Hocke AC, Kessler M, Zobel CM, Witzenrath M and Nouailles G. Advanced in vitro models for pneumonia research.

63rd Congress of the DGP, 29.03 – 01.04.2023, Düsseldorf

Pfeiffer L, Weißfuß C, Behrendt U, Wienhold S, Gutbier B, Krishnamoorthy G, Witzenrath M, Nouailles G, Goekeri C, Pennitz P. Approaching xeno-free cultivation and utilization of pulmonary pathogens in vitro.

International Conference of the SFB-TR 84, 8. – 10.09.2022, Berlin

Pfeiffer L, Weißfuß C, Behrendt U, Wienhold S, Gutbier B, Krishnamoorthy G, Witzenrath M, Nouailles G, Goekeri C, Pennitz P. – Approaching xeno-free cultivation and utilization of pulmonary pathogens in vitro.

2nd Summer School on „infection Biology“ of the Universitöt Greifswald and FLI, 26. – 28.09.2022, Greifswald

Pfeiffer L, Weißfuß C, Behrendt U, Wienhold S, Gutbier B, Krishnamoorthy G, Witzenrath M, Nouailles G, Goekeri C, Pennitz P. – Approaching xeno-free cultivation of pulmonary pathogens in vitro.

Project Links

https://charite3r.charite.de/

NoVAP Lab Bundeswehr Krankenhaus

Host immunity – booster or dampener of phage therapy efficacy

Project members

Geraldine Nouailles

Chantal Weißfuß

Ulrike Behrendt

Main cooperation partners

Martin Witzenrath

MAPVAP consortium

Project-related publications

Bürkle M, Korf IHE, Lippegaus A, Krautwurst S, Rohde C, Weissfuss C, Nouailles G, Tene XM, Gaborieau B, Ghigo JM, Ricard JD, Hocke AC, Papenfort K, Debarbieux L, Witzenrath M, Wienhold SM, Krishnamoorthy G. Phage-phage competition and biofilms affect interactions between two virulent bacteriophages and Pseudomonas aeruginosa. ISME J. 2025:wraf065.

Weissfuss C, Li J, Behrendt U, Hoffmann K, Bürkle M, Krishnamoorthy G, Korf IHE, Rohde C, Gaborieau B, Debarbieux L, Ricard J-D, Witzenrath M, Felten M, Nouailles G. Adjunctive phage therapy improves antibiotic treatment of Ventilator-Associated-Pneumonia with Pseudomonas aeruginosa. Preprint. 2024

Weissfuss C, Wienhold SM, Bürkle M, Gaborieau B, Bushe J, Behrendt U, Bischoff R, Korf IHE, Wienecke S, Dannheim A, Ziehr H, Rohde C, Gruber AD, Ricard JD, Debarbieux L, Witzenrath M, Nouailles G. Repetitive Exposure to Bacteriophage Cocktails against Pseudomonas aeruginosa or Escherichia coli Provokes Marginal Humoral Immunity in Naïve Mice. Viruses. 2023;15(2):387

Wienhold SM, Brack MC, Nouailles G, Krishnamoorthy G, Korf IHE, Seitz C, Wienecke S, Dietert K, Gurtner C, Kershaw O, Gruber AD, Ross A, Ziehr H, Rohde M, Neudecker J, Lienau J, Suttorp N, Hippenstiel S, Hocke AC, Rohde C, Witzenrath M. Preclinical Assessment of Bacteriophage Therapy against Experimental Acinetobacter baumannii Lung Infection. Viruses. 2021;14(1):33.

Conference Papers

Lung Science Conference (ERS). 20. – 23 March 2025, Estoril, Portugal.

Weissfuss C, Hoffmann K, Behrendt U, Bürkle M, Debarbieux L, Ricard J-D, Witzenrath M, Zobel CM, Nouailles G. Immunophage Synergy: Supporting Innate Immunity with Phages to Combat Bacterial Pneumonia.

Joint DGP/DZL Academy Lung Science Meeting. 22. – 23 November 2024, Freiburg.

Weissfuß C, Li J, Behrendt U, Hoffmann K, Bürkle M, Krishnamoorthy G, Korf IHE, Rohde C, Gaborieau B, Debarbieux L, Ricard J-D, Witzenrath M, Felten M, Nouailles G. – Adjunctive phage therapy with antibiotics improves Ventilator-Associated-Pneumonia with Pseudomonas aeruginosa.

7th joint conference “Joint Microbiology & Infection Conference 2024” of the DGHM and VAAM. 02. – 05. June 2024, Würzburg.

Weißfuß C, Li J, Behrendt U, Bürkle M, Gaborieau B, Korf I, Wienecke S, Dannheim A, Ziehr H, Rohde C, Gruber A D, Gopinath K, Ricard J-D, Debarbieux L, Witzenrath M, Felten M, and Nouailles G. – Combining a phage cocktail with antibiotics improves recovery from Pseudomonas-induced VAP in mice.

64. Congress of the German Respiratory Society (DGP) Section Cell Biology. 20. – 23. March 2024, Mannheim.

Weißfuß C, Li J, Behrendt U, Bürkle M, Gaborieau B, Korf I, Wienecke S, Dannheim A, Ziehr H, Rohde C, Gruber A D, Gopinath K, Ricard J-D, Debarbieux L, Witzenrath M, Felten M, and Nouailles G. – Combining a phage cocktail with antibiotics improves recovery from Pseudomonas-induced VAP in mice.

Herbsttagung 2023 of the German Respiratory Society (DGP) Section Cell Biology. 10. – 11. November 2023, Essen.

Weißfuß C, Li J, Behrendt U, Bürkle M, Gaborieau B, Korf I, Wienecke S, Dannheim A, Ziehr H, Rohde C, Gruber A D, Gopinath K, Ricard J-D, Debarbieux L, Witzenrath M, Felten M, and Nouailles G. – Combining a phage cocktail with antibiotics improves recovery from Pseudomonas-induced VAP in mice.

Herbsttagung 2021 of the German Respiratory Society (DGP) Section Cell Biology. 12. – 13. November 2021, Hannover. (shared 1st place)

Weißfuß C, Wienhold S-M, Bürkle M, Gaborieau B, Bushe J, Behrendt U, Bischoff R, Korf I, Wienecke S, Dannheim A,  Ziehr H, Rohde C, Gruber A D, Ricard J-D, Debarbieux L, Witzenrath M, and Nouailles G. – Repetitive exposure to bacteriophage cocktails against multidrug-resistant P. aeruginosa or E. coli does not induce pronounced immunity in naïve mice.

62. Congress of the German Respiratory Society (DGP) Section Cell Biology. 25. – 28. May 2022, Leipzig. (Best of Herbsttagung 2021)

Weißfuß C, Wienhold S-M, Bürkle M, Gaborieau B, Bushe J, Behrendt U, Bischoff R, Korf I, Wienecke S, Dannheim A,  Ziehr H, Rohde C, Gruber A D, Ricard J-D, Debarbieux L, Witzenrath M, and Nouailles G. – Repetitive exposure to bacteriophage cocktails against multidrug-resistant P. aeruginosa or E. coli does not induce pronounced immunity in naïve mice.

Project Links

Combination of in vivo phage therapy data with in silico model highlights key parameters for pneumonia treatment efficacy. Cell Reports. May 2022;39(17).

Project site at the BMBF

Project site at the Institut Pasteur

Protective mode of action of immunoglobulin (Trimodulin) therapy in pneumonia. 

Project members

Geraldine Nouailles

Romina Bischoff

Kerstin Linke

Alexander Taylor (Alumni)

Main cooperation partners

Martin Witzenrath         

 Biotest AG

 Achim Gruber

Project-related publications

Jahn K, Handtke S, Palankar R, Weißmüller S, Nouailles G, Kohler TP, Wesche J, Rohde M, Heinz C, Aschenbrenner AF, Wolff M, Schüttrumpf J, Witzenrath M, Hammerschmidt S, Greinacher A. Pneumolysin induces platelet destruction, not platelet activation, which can be prevented by immunoglobulin preparations in vitro. Blood Adv. 2020;4(24):6315-6326.

Project Links

Description at clinicaltrials.gov

Description at biotest.com

In vitro, in vivo and real-world data turned into mathematical models of pneumonia and cardiovascular complications thereof.

Project members

Geraldine Nouailles

Peter Pennitz

Main cooperation partners

Martin Witzenrath, Markus Brack and team

Markus Scholz and team

CAPSyS consortium

Berlin Hamster consortium

Project-related publications

Schirm S, Nouailles G, Kirsten H, Trimpert J, Wyler E, Teixeira Alves LG, Landthaler M, Ahnert P, Suttorp N, Witzenrath M, Scholz M. A biomathematical model of SARS-CoV-2 in Syrian hamsters. Sci Rep. 2024;14(1):30541.

Ramezani Rad P, Nageswaran V, Peters L, Reinshagen L, Roessler J, Simmons S, Asmus E, Wittig C, Brack MC, Nouailles G, van der Vorst EPC, Maas SL, Sonnenschein K, Verhaar BJH, Szulcek R, Witzenrath M, Landmesser U, Kuebler WM, Haghikia A. Pneumonia Induced Rise in Glucagon Promotes Endothelial Damage and Thrombogenicity. Circ Res. 2024;135(11):1116-1118.

Friedrich VD*, Pennitz P*, Wyler E, Adler JM, Postmus D, Müller K, Teixeira Alves LG, Prigann J, Pott F, Vladimirova D, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Saliba AE, Scholz M, Witzenrath M, Trimpert J, Kirsten H#, Nouailles G#. Neural network-assisted humanisation of COVID-19 hamster transcriptomic data reveals matching severity states in human disease. EBioMedicine. 2024;108:105312. *,#: equal contribution.

Peidli S*, Nouailles G*, Wyler E, Adler JM, Kunder S, Voß A, Kazmierski J, Pott F, Pennitz P, Postmus D, Teixeira Alves LG, Goffinet C, Gruber AD, Blüthgen N, Witzenrath M, Trimpert J#, Landthaler M#, Praktiknjo SD#. Single-cell-resolved interspecies comparison shows a shared inflammatory axis and a dominant neutrophil-endothelial program in severe COVID-19. Cell Rep. 2024;43(6):114328. *,#: equal contribution.

Wyler E, Adler JM, Eschke K, Teixeira Alves G, Peidli S, Pott F, Kazmierski J, Michalick L, Kershaw O, Bushe J, Andreotti S, Pennitz P, Abdelgawad A, Postmus D, Goffinet C, Kreye J, Reincke SM, Prüss H, Blüthgen N, Gruber AD, Kuebler WM, Witzenrath M, Landthaler M, Nouailles G, Trimpert J. Key benefits of dexamethasone and antibody treatment in COVID-19 hamster models revealed by single-cell transcriptomics. Mol Ther. 2022;30(5):1952-1965.

Nouailles G, Wyler E, Pennitz P, Postmus D, Vladimirova D, Kazmierski J, Pott F, Dietert K, Muelleder M, Farztdinov V, Obermayer B, Wienhold SM, Andreotti S, Hoefler T, Sawitzki B, Drosten C, Sander LE, Suttorp N, Ralser M, Beule D, Gruber AD, Goffinet C, Landthaler M, Trimpert J, Witzenrath M. Temporal omics analysis in Syrian hamsters unravel cellular effector responses to moderate COVID-19. Nat Commun. 2021;12(1):4869.

Schirm S, Haghikia A, Brack M, Ahnert P, Nouailles G, Suttorp N, Loeffler M, Witzenrath M, Scholz M. A biomathematical model of atherosclerosis in mice. PLoS One. 2022;17(8):e0272079.

Schirm S, Ahnert P, Berger S, Nouailles G, Wienhold SM, Müller-Redetzky H, Suttorp N, Loeffler M, Witzenrath M, Scholz M. A biomathematical model of immune response and barrier function in mice with pneumococcal lung infection. PLoS One. 2020;15(12):e0243147.

Berger S, Goekeri C, Gupta SK, Vera J, Dietert K, Behrendt U, Lienau J, Wienhold SM, Gruber AD, Suttorp N, Witzenrath M, Nouailles G. Delay in antibiotic therapy results in fatal disease outcome in murine pneumococcal pneumonia. Crit Care. 2018;22(1):287.

Schirm S, Ahnert P, Wienhold S, Mueller-Redetzky H, Nouailles-Kursar G, Loeffler M, Witzenrath M, Scholz M. A Biomathematical Model of Pneumococcal Lung Infection and Antibiotic Treatment in Mice. PLoS One. 2016;11(5):e0156047.

Conference papers

e:Med Meeting 2024, 21 – 23.11.2024, Hamburg

Brack M, Steinhorst R, Pennitz P, Mueller K, Nouailles G, Teixeira Alves LG, Wyler E, Witzenrath M, Scholz M, Kirsten H. From Lung to Heart: scRNA-seq Illuminates Post-Pneumonia Cardiovascular Risks.

American Thoracic Society 2024 International Conference, 17 – 22.05.2024, San Diego, USA

Melnikov A, Solymosi PD, Pennitz P, Schupp JC, Nouailles G, Perret PL, Gallo K, Hegemann N, Nambiar Veetil N, Klepetko W, Sinn K, Erfinanda L, Simmons S, Kucherenko MM, Knosalla C, Preissner R, Kwapiszewska G, Witzenrath M, Kuebler WM. Cellular Mechanisms Underlying the Pathogenetic Role of the Primary Cilium in Pulmonary Arterial Hypertension (abstract). Am J Respir Crit Care Med 2024;209:A6790. DOI: https://doi.org/10.1164/ajrccm-conference.2024.209.1_MeetingAbstracts.A6790

e:Med Meeting 2023, 10 – 11.10.2023, Berlin

Pennitz P*, Friedrich VD*, Wyler E, Adler JM, Postmus D, Andreotti S, Teixeira Alves LG, Kazmierski J, Pott F, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Trimpert J, Scholz M, Witzenrath M, Kirsten H#, Nouailles G#. Interspecies analysis to dissect transcriptomic signatures of humans and hamsters in COVID-19.

e:Med Meeting 2022, 28 – 30.11.2022, Heidelberg

Pennitz P*, Kirsten H*, Friedrich VD, Wyler E, Goekeri C, Obermayer B, Heinz GA, Mashreghi MF, Büttner M, Trimpert J, Landthaler M, Suttorp N, Hocke AC, Hippenstiel S, Tönnies M, Scholz M, Kuebler WM, Witzenrath M, Hoenzke K, Nouailles G. Integrational approaches for cross-species analysis of lung pathologies at single-cell resolution.

Friedrich VD*, Pennitz P*, Wyler E, Adler JM, Postmus D, Andreotti S, Teixeira Alves LG, Kazmierski J, Pott F, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Trimpert J, Scholz M, Witzenrath M, Kirsten H#, Nouailles G#. Interspecies analysis to dissect cellular transcriptomic signatures of humans and hamsters in COVID-19.

SCOG Conference “Single Cell Omics in Clinical Applications”, 07 – 09.11.2022, Bonn.

Pennitz P *, Friedrich VD *, Wyler E, Adler JM, Postmus D, Andreotti S, Teixeira-Alves LG, Kazmierski J, Pott F, Hoefler T, Goekeri C, Landthaler M, Goffinet C, Trimpert J, Scholz M, Witzenrath M, Kirsten H#, Nouailles G#. Interspecies analysis to dissect cellular transcriptomic signatures of humans and hamsters in COVID-19.

21st International Conference on Systems Biology, 08.-12.10.2022, Berlin.

Pennitz P*, Kirsten H*, Friedrich VD, Wyler E, Goekeri C, Obermayer B, Heinz GA, Mashreghi MF, Büttner M, Trimpert J, Landthaler M, Suttorp N, Hocke AC, Hippenstiel S, Tönnies M, Scholz M, Kuebler WM, Witzenrath M, Hoenzke K, Nouailles G. Integrational approaches for cross-species analysis of lung pathologies at single-cell resolution.

e:Med Meeting 2021, 20 – 22.09.2021, online.

Pennitz P, Kirsten H, Goekeri C, Behrendt U, Gutbier B, Heinz G, Mashreghi MF, Wienhold SM, Witzenrath M, Scholz M, Nouailles G. Single-cell RNA-seq for a murine pneumonia lung atlas improving pathomechanistical understanding of community acquired pneumonia.

Project Links

CAPSyS at the BMBF

CAPSyS Homepage

CAPSyS at Systems Medicine

SYMPATH at Systems Medicine

SYMPATH at the BMBF

Press Releases

How AI is helping to bridge the research gap between animals and humans (idw, 25.09.2024)

  • Go to page 1
  • Go to page 2
  • Go to Next Page »

Primary Sidebar

  • Home
  • News
  • Research
  • Funding
  • Publications
  • Team
    • Alumni
  • Career

Contact

Dr. Geraldine Nouailles
Department of Infectious Diseases and Respiratory Medicine
Charité – Universitätsmedizin Berlin
Charitéplatz 1
10117 Berlin
geraldine.nouailles@charite.de

Powered by the Genesis Framework | Impressum | Datenschutzerklärung